Description
The Real-time PCR System YR06248 // YR06249 is engineered with cutting-edge signal gathering and spectrum separation technology complemented by a unique temperature control mechanism. This state-of-the-art system enhances detection sensitivity, offers multi-color crosstalk correction, and ensures excellent temperature uniformity and precision. Available with either a 4-channel or 5-channel configuration, it is highly compatible with both qPCR dye-based and probe-based chemistries, making multiplex PCR applications seamless. With support for standard analysis methods such as absolute quantification, genotyping, HRM, MMCA, and gene expression analysis, this instrument is an invaluable asset for any laboratory.
Market Price
The market price for a real-time PCR system like the YR06248 // YR06249 typically ranges between $22,500 and $23,700 USD. This competitive price range positions it as an accessible choice for laboratories seeking high sensitivity and accuracy in their quantitative PCR processes.
Frequently Asked Questions
What is the sample capacity of the Real-time PCR System YR06248 // YR06249? The system can accommodate 8*0.1mL PCR strip*12, 96*0.1mL individual tubes, or a 0.1mL PCR plate.
What are the compatible chemistries? It supports a wide range of qPCR dye-based and probe-based chemistries.
Advantages and Disadvantages
The main advantages of the Real-time PCR System YR06248 // YR06249 include its fast and precise thermal cycling, innovative optical design, and excellent reproducibility. Users will appreciate its dynamic range of up to 10-log, offering remarkable resolution in melting curve analysis. However, laboratories with limited budgets might find the initial investment slightly higher, although the system’s performance and reliability justify the cost.
Use of the Product in the Field
This PCR system is predominantly used in laboratories for quantitative PCR experiments, including but not limited to gene expression analysis and genotyping. Its robust design caters to comprehensive applications in research institutions, clinical settings, and academic environments worldwide.
Recommendations
To maximize efficiency and lifespan, ensure regular maintenance and calibration of the system. Handle reagents and samples in a contamination-free environment to uphold the system’s high sensitivity levels. For price inquiries and more detailed specifications, consider requesting a quotation through Kalstein Plus.
Features
- Fast and Precise Thermal Cycling
- Innovative Optic Design
- Excellent Reproducibility
- Up to 10-log dynamic range
- Melting Curve Analysis
- High Resolution
Technical Specifications
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
